In Situ Cell Death Detection Kit, TMR red

TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

Experiment
TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma
Product
In Situ Cell Death Detection Kit, TMR red from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
Fix cell samples with Fixation solution for 1 h.

Incubate in Permeabilisation solution (0.1% Triton X-100 in PBS) for 2 min on ice.

Label and incubate for 60 min at +37°C in a humidified atmosphere in the dark
Downstream tips
If low labelling is found, reduce fixation time or try 2% buffered paraformaldehyde

Publication protocol

The In situ Cell Death Detection Kit-TMR (Roche) was used to detect cell apoptosis following the manufacture’s instruction. The pictures were taken under a fluorescent microscope and the percentage of positive cells was calculated using Image J software (NIH). Four fields were randomly chosen from each well of 12-well plates for the analysis.

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma using In Situ Cell Death Detection Kit, TMR red from Sigma-Aldrich.

Paper title
Ginsenoside metabolite compound K enhances the efficacy of cisplatin in lung cancer cells
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for In Situ Cell Death Detection Kit, TMR red below.

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