Cytotoxicity Detection Kit (LDH)

Live / Dead assay bacteria - Pseudomonas aeruginosa

Experiment
Live / Dead assay bacteria - Pseudomonas aeruginosa
Product
Cytotoxicity Detection Kit (LDH) from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
Incubate the cells in an incubator (37°C, 5% CO2, 90% humidity

Publication protocol

Percent cytotoxicity for each corneal isolate was determined by measuring the amount of lactate dehydrogenase released into the media by dead or dying host cells after bacterial exposure using a cytotoxicity detection kit (Roche Diagnostics, Indianapolis, IN). Values were compared to a positive control, P. aeruginosa clinical isolate 6206. In some experiments, invasive control 6294 had slightly lower LDH values than the media (baseline) control. Negative values for percent cytotoxicity corresponded to strains that were not cytotoxic and had negative LDH values after the media control had been included in the calculation. Values greater than one corresponded to invasiveness or cytotoxicity greater than the respective positive control.

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Discussion

Discussion

4 years ago

Author: M. Daecher Germany

Live/dead assay Bacteria

Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay bacteria - Pseudomonas aeruginosa using Cytotoxicity Detection Kit (LDH) from Sigma-Aldrich.

Paper title
Association between Cytotoxic and Invasive and Clinical Outcomes in Bacterial Keratitis
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Cytotoxicity Detection Kit (LDH) below.

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