EpiTect Methyl II PCR Assays

DNA methylation profiling Gene specific profiling - MCF-7 FOXA2

Experiment
DNA methylation profiling Gene specific profiling - MCF-7 FOXA2
Product
EpiTect Methyl II PCR Assays from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Epitect methyl qPCR arrays
To identify relevant gene promoter methylation we used EpiTect Methyl qPCR Arrays (SA Biosciences, Qiagen, Hilden, Germany) as a screening method. We analyzed 96 cancer-related genes from four pathways including stem cell transcription factors, homeobox genes, wnt signaling, and epithelial to mesenchymal transition (EMT). Assays were performed according to the manufacturer’s instructions. Results were displayed as percentage of unmethylated and methylated fraction.



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Reviews

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Discussion

Discussion

5 years ago

Author: Jody Hancock Canada

How can I preserve leukocytes for DNA methylation profiling?

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - MCF-7 FOXA2 using EpiTect Methyl II PCR Assays from Qiagen.

Paper title
Genetic and epigenetic analysis of putative breast cancer stem cell models
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Manufacturer protocol

Download the product protocol from Qiagen for EpiTect Methyl II PCR Assays below.

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Videos

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