EpiTect Bisulfite Kit

DNA methylation profiling Gene specific profiling - HepG2 Fibrinogen

Experiment
DNA methylation profiling Gene specific profiling - HepG2 Fibrinogen
Product
EpiTect Bisulfite Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Bisulfite DNA methylation analysis
Genomic DNA from cell lines and human primary cells was isolated using DNeasy Blood & Tissue kit (QIAGEN, Hilden, Germany). Genomic DNA from animal tissues was isolated using proteinase/phenol extraction. Bisulfite DNA conversion was performed using the EpiTect Bisulfite Kit (QIAGEN, Hilden, Germany). PCRs on bisulfite treated DNA were performed with primers listed in using the KAPA2G Robust HotStart ReadyMix (Kapa Bioystems, Boston, Massachusetts, United States). Methylation of individual CpG sites was detected by direct sequencing using T-Tracking (QIAGEN, Hilden, Germany) [].

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Reviews

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Discussion

Discussion

5 years ago

Author: Jody Hancock Canada

How can I preserve leukocytes for DNA methylation profiling?

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - HepG2 Fibrinogen using EpiTect Bisulfite Kit from Qiagen.

Paper title
DNA Methylation Profiling of the Fibrinogen Gene Landscape in Human Cells and during Mouse and Zebrafish Development
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Manufacturer protocol

Download the product protocol from Qiagen for EpiTect Bisulfite Kit below.

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Videos

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