MICROBExpress™ Bacterial mRNA Enrichment Kit

RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus

Experiment
RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus
Product
MICROBExpress™ Bacterial mRNA Enrichment Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
This kit removes 16S and 23S rRNA so that only mRNA remains. It is not suitable for only RNA isolation.

Requires a magnetic stand

Publication protocol

A total of 4 ug RNA was subjected to rRNA depletion using the Illumina Ribo-Zero rRNA Removal Kit (Bacteria), Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit and the Life Technologies RiboMinus Transcriptome Isolation Kit, Bacteria, according to the manufacturers’ protocols. RNA quantity and quality post-depletion was assessed using the Agilent 2100 Bioanalyzer instrument as described above. In order to ensure valid comparison of the rRNA depletion methods, the procedures were performed on aliquots of the same input RNA sample, with the comparisons repeated using biological triplicates.

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Reviews

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Discussion

Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for MICROBExpress™ Bacterial mRNA Enrichment Kit below.

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