MethylFlash™ Methylated DNA Quantification Kit

DNA quantification Human - SH-SY5Y

Experiment
DNA quantification Human - SH-SY5Y
Product
MethylFlash™ Methylated DNA Quantification Kit from Epigentek
Manufacturer
Epigentek

Protocol tips

Upstream tips
Do not allow pipette tip to touch the outer edges or inner sides of the wells to prevent solutions from sticking to the surface

Publication protocol

DNA Methylation Analysis.
DNA was isolated from SH-SY5Y cell culture after various pretreatments, and any contaminating RNA was removed by RNase treatment. Assessment of global DNA methylation status was accomplished as described previously (Hodgson et al., 2013) using the MethylFlash Methylated DNA Quantification Kit from Epigentek (see the manufacturer’s website for full protocol). The methylated fraction of DNA was identified using 5-methylcytosine monoclonal antibodies and quantified by an enzyme-linked immunosorbent assay–like reaction. The levels of methylated DNA were calculated using the optical density intensity on a microplate reader at 450 nm.

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Discussion

Discussion

5 years ago

Author: Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA quantification Human - SH-SY5Y using MethylFlash™ Methylated DNA Quantification Kit from Epigentek.

Paper title
Morphine Induces Redox-Based Changes in Global DNA Methylation and Retrotransposon Transcription by Inhibition of Excitatory Amino Acid Transporter Type 3–Mediated Cysteine Uptake
Full paper
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Manufacturer protocol

Download the product protocol from Epigentek for MethylFlash™ Methylated DNA Quantification Kit below.

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Videos

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