Qubit dsDNA HS Assay Kit

DNA quantification Human - MDA-MB-231

Experiment
DNA quantification Human - MDA-MB-231
Product
Qubit dsDNA HS Assay Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Do not label the side of the tube as this could interfere with the sample read.

Publication protocol

DNA quantification and mtDNA purity assessment
All DNA extracts were quantified using the Qubit dsDNA HS assay kit (Life Technologies) according to the suppliers’ protocol. Purity of mtDNA was assessed in duplicate runs of a multiplex qPCR assay targeting a nuclear and a mitochondrial encoded gene to calculate the ratio of mtDNA molecules opposed to nDNA molecules by the relative quantitation method (2ΔCq) as described before44. The percentage of mtDNA in the DNA extract was quantified (eq. 1) based on the ratio mtDNA:nDNA molecules and the sizes of the mitochondrial reference genome (16,569 base pairs, NC_012920) and complete reference genome (haploid 3,088,269,805 base pairs, GRCh38).

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Discussion

Discussion

5 years ago

Author: Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA quantification Human - MDA-MB-231 using Qubit dsDNA HS Assay Kit from Thermo Fisher Scientific.

Paper title
Sensitive detection of mitochondrial DNA variants for analysis of mitochondrial DNA-enriched extracts from frozen tumor tissue
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Qubit dsDNA HS Assay Kit below.

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