MethylFlash Methylated DNA 5-mC Quantification Kit

DNA quantification Human - HEK 293

Experiment
DNA quantification Human - HEK 293
Product
MethylFlash Methylated DNA 5-mC Quantification Kit from Epigentek
Manufacturer
Epigentek

Protocol tips

Protocol tips
Do not allow pipette tip to touch the outer edges or inner sides of the wells to prevent solutions from sticking to the surface

Publication protocol

Quantification of DNA methylation
Total DNA from the cells upon specific treatments was extracted and purified with the DNeasy kit (Qiagen), followed by quality control with a NanoDrop spectrophotometer (Thermo Scientific). The level of global DNA methylation was quantified with the MethylFlash methylated DNA quantification kit (Epigentek). The methylation assay is a 96-well-plate-based colorimetric immunoassay. In each well 100 ng of DNA was attached to the bottom surface and the DNA methylation marks were labeled with monoclonal antibodies. The developed color signals were normalized to that from a standard DNA sample with known methylation percentage. The promoter methylation of selected cancer miRNAs was assessed by a PCR-based EpiTect Methyl II array (EAHS-591Z, Qiagen), following the protocol provided by the manufacturer.

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Reviews

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Discussion

Discussion

5 years ago

Author: Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA quantification Human - HEK 293 using MethylFlash Methylated DNA 5-mC Quantification Kit from Epigentek.

Paper title
Epigenetic toxicity of trichloroethylene: a single-molecule perspective
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Manufacturer protocol

Download the product protocol from Epigentek for MethylFlash Methylated DNA 5-mC Quantification Kit below.

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Videos

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