7-AAD (7-Aminoactinomycin D)

DNA quantification Human - BMDM

Experiment
DNA quantification Human - BMDM
Product
7-AAD (7-Aminoactinomycin D) from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
incubate cells on ice only

Publication protocol

Cell cycle analysis
BMDM were infected with for 16 hr as previously described or treated with Bleocin with or without IFN-β, as described above. Cells were then pulsed with BrdU for 30 min using the BrdU-FITC or –APC kits per the manufacturer’s instructions. DNA content was assessed by 7AAD (BD) and data were acquired on a FACSCalibur or BD LSR II (BD Biosciences) and were analyzed with FlowJo software (TreeStar).

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Reviews

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Discussion

Discussion

5 years ago

Author: Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA quantification Human - BMDM using 7-AAD (7-Aminoactinomycin D) from Thermo Fisher Scientific.

Paper title
A type I IFN-dependent DNA damage response regulates the genetic program and inflammasome activation in macrophages
Full paper
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for 7-AAD (7-Aminoactinomycin D) below.

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Videos

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