Qubit dsDNA HS Assay Kit

DNA quantification Human - BMDM

Experiment
DNA quantification Human - BMDM
Product
Qubit dsDNA HS Assay Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Do not label the side of the tube as this could interfere with the sample read.

Publication protocol

High throughput sequencing
Single read library preparation and high throughput single read sequencing for 36 cycles was carried out on an Illumina Genome Analyser IIx according to the manufacturer’s protocols (Illumina) with some modifications. Each immunoprecipitated sample for library preparation was the product of five separate technical replicates of immunoprecipitation pooled together and purified using Qiagen MinElute columns and quantified using the hsDNA Qubit assay (Invitrogen, Life Technologies, California, USA). A 1:20 dilution of Illumina adaptors was used at the adaptor ligation step to avoid adaptor dimer formation and a 1:2 primer dilution used to prevent dimerization during the PCR amplification stage. The samples were quantified using the hsDNA Qubit assay (Invitrogen, Life Technologies, California, USA) and the size range analysed using a HS DNA chip on a 2100 Bioanalyser (Agilent Technologies, West Lothian, UK) prior to submission for qPCR analysis and cluster generation and sequencing by the CSC/IC Genome Core facility.

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Reviews

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Discussion

Discussion

5 years ago

Author: Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA quantification Human - BMDM using Qubit dsDNA HS Assay Kit from Thermo Fisher Scientific.

Paper title
Combined ChIP-Seq and transcriptome analysis identifies AP-1/JunD as a primary regulator of oxidative stress and IL-1β synthesis in macrophages
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Qubit dsDNA HS Assay Kit below.

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Videos

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