CM-H2DCFDA (General Oxidative Stress Indicator)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Do not dissolve products until immediately prior to use	- Cells were measure for ROS levels after 24h of platting.

Upstream tips
- Do not dissolve products until immediately prior to use
Protocol tips
- Cells were measure for ROS levels after 24h of platting.

Publication protocol

Intracellular ROS levels were measured at 24 hours post-plating using CM-H2DCFDA (Thermo Fisher Scientific) as described previously [31]. Each of 3 independent experiments included a negative control of unlabeled cells to verify staining specificity. Results were normalized to cell number obtained by staining with crystal violet.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for CM-H2DCFDA (General Oxidative Stress Indicator) below.

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