TUNEL Assay Kit - BrdU-Red

TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells

Experiment
TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells
Product
TUNEL Assay Kit - BrdU-Red from Abcam
Manufacturer
Abcam

Protocol tips

Upstream tips
Grow cells without any growth factors for 24 h.

Treat cells with Cur or SLCP (25 μM) for 24–72 h
Protocol tips
fix cells with 4% paraformaldehyde for 15 min,
Downstream tips
View slides as soon as possible. Analyze cells within 3 hours of
staining.

Publication protocol

The TUNEL staining was performed as per manufacturer's instructions [28, 35]. Briefly, U-87MG cells were grown on coverslips in EMEM, without any growth factors for 24 h and then they were treated with Cur or SLCP (25 μM) for 24–72 h. Following treatment, the cells were fixed with 4% paraformaldehyde for 15 min, and then TUNEL staining was performed [28, 35]. Finally, the cells were counterstained with Hoechst 3342 for 5 min at room temperature in the dark and washed thoroughly with distilled water, after which they were mounted on a glass slide with antifading medium. The cells were observed under a fluorescent microscope (Leica, Germany), using appropriate filters (ex/em: 488/576). The red fluorescent signal indicated TUNEL-positive cells. The number of total cells and that of TUNEL-positive cells were counted and expressed as a percentage of the total cell count. Almost two thousand total cells were counted in each group of randomly selected microscopic fields from three independent experiments to obtain a mean value.

Full paper   Login or join for free to view the full paper.

Reviews

TUNEL Assay Kit - BrdU-Red from Abcam has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells using TUNEL Assay Kit - BrdU-Red from Abcam.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Abcam for TUNEL Assay Kit - BrdU-Red below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells using TUNEL Assay Kit - BrdU-Red from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms