Bambi siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA Sense-GCAAGCAGAGCUCAGUAAUTT siRNA AntiSense-AUUACUGAGCUCUGCUUGCTT Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. Replace medium after 6h. Incubate cells for 1-3 days at 37°C and quantify.

Protocol tips
siRNA Sense-GCAAGCAGAGCUCAGUAAUTT siRNA AntiSense-AUUACUGAGCUCUGCUUGCTT Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. Replace medium after 6h. Incubate cells for 1-3 days at 37°C and quantify.

Publication protocol

BAMBI siRNA and a scrambled negative control (NC) were designed and purchased from Invitrogen (Table 1). When the cell density reached 70%, the C2C12 myoblasts were subjected to starvation in Opti-MEM® I reduced serum medium (Invitrogen) for 4 h prior to transfection. Lipofectamine® 2000 transfection reagent (Invitrogen) and siRNA or NC were gently mixed according to the manufacturer’s protocol, and then added to the culture supernatant. After 6 h of incubation, Opti-MEM I medium was replaced with fresh DM.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Bambi siRNA below.

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