TruSeq RNA Library Prep Kit v2

RNA sequencing Mouse - ESCs (Embryonic Stem Cells)

Experiment
RNA sequencing Mouse - ESCs (Embryonic Stem Cells)
Product
TruSeq RNA Library Prep Kit v2 from Illumina
Manufacturer
Illumina

Protocol tips

Protocol tips
0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)

Publication protocol

Total RNA (1 μg) was used for deep-sequencing library construction following the instructions of the TruSeq RNA sample preparation kit (Illumina). The final quality-ensured libraries were sequenced on a HiSeq 2000 sequencer generating 100 bp paired-end reads.

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Reviews

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Discussion

Discussion

5 years ago

Author: Panagiotis Karagiannis Greece

Using RNA from formalin fixed paraffin embedded tissue sections for sequencing

Hello there! I have some slides with mouse lung tumors and I would like to do rna sequencing. Will I be able to extract enough/good quality rna from RNA from formalin fixed paraffin embedded tissue sections for sequencing to be successful?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA sequencing Mouse - ESCs (Embryonic Stem Cells) using TruSeq RNA Library Prep Kit v2 from Illumina.

Paper title
Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos
Full paper
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Manufacturer protocol

Download the product protocol from Illumina for TruSeq RNA Library Prep Kit v2 below.

Download PDF Download manufacturer protocol

Videos

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