Upstream tips |
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Seed 1×10^4 cells/well in 24-well plate |
Protocol tips |
Transfect cells with 200 ng of the luciferase construct plasmid and 50 ng of pMIR-REPORT β-gal |
pMIR-REPORT™ miRNA Expression Reporter Vector System from Thermo Fisher Scientific has not yet been reviewed for this experiment
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Discussion
5 years ago
Author: J.Han
I am planning on running reporter assays on stem cells and I would like to know which transfection reagent would work better?
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Download the product protocol from Thermo Fisher Scientific for pMIR-REPORT™ miRNA Expression Reporter Vector System below.
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