TRI Reagent™ Solution

Protein isolation Bacteria - Vibrio cholerae

Experiment
Protein isolation Bacteria - Vibrio cholerae
Product
TRI Reagent™ Solution from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- The protein is precipitated by pre-chilled acetone at ice for 15 minutes.

Publication protocol

Protein extraction using TRI-Reagent kit (Molecular Research Center, Cincinnati, US) was done as recommended by the supplier. Briefly, 1mL of TRI-reagent and chloroform was added sequentially per 5x105 of cells / 0.05g of pus and centrifuged at 12 000g at 4oC for 15 minutes to separate into DNA, RNA and protein layers. Instead of following the manufacturer’s protocol, we modified the protein precipitation method where the protein was precipitated by pre-chilled acetone at ice for 15 minutes.

Full paper   Login or join for free to view the full paper.

Reviews

TRI Reagent™ Solution from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Hollie Fowler United Kingdom

How can I deal with my pellet being too viscous?

I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Bacteria - Vibrio cholerae using TRI Reagent™ Solution from Thermo Fisher Scientific.

Paper title
Optimal protein extraction methods from diverse sample types for protein profiling by using Two-Dimensional Electrophoresis (2DE).
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRI Reagent™ Solution below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Protein isolation Bacteria - Vibrio cholerae using TRI Reagent™ Solution from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms