MagMAX™ Total Nucleic Acid Isolation Kit

RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Experiment
RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis
Product
MagMAX™ Total Nucleic Acid Isolation Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Kit relies on bead-beating and magnetism, so adequate lab equipment is required, check the manual for required materials.

Publication protocol

a commercial kit and protocol was followed (MagMAX™ Total Nucleic Acid Isolation Kit, 96 well plate format, Thermo Fisher Scientific).

Full paper   Login or join for free to view the full paper.

Reviews

MagMAX™ Total Nucleic Acid Isolation Kit from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis using MagMAX™ Total Nucleic Acid Isolation Kit from Thermo Fisher Scientific.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for MagMAX™ Total Nucleic Acid Isolation Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis using MagMAX™ Total Nucleic Acid Isolation Kit from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms