RNeasy Plus Mini Kit

RNA isolation / purification Cells - immortalized Mutu-1

Experiment
RNA isolation / purification Cells - immortalized Mutu-1
Product
RNeasy Plus Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

RNA was isolated from the EBV-positive BL cell lines Mutu I, Mutu III, Kem I, and Kem III and primary tonsillar naive and GC B cells using the RNeasy Plus minikit (Qiagen) according to the manufacturer's instructions

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized Mutu-1 using RNeasy Plus Mini Kit from Qiagen.

Paper title
Restricted TET2 Expression in Germinal Center Type B Cells Promotes Stringent Epstein-Barr Virus Latency
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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