RNeasy Micro Kit

RNA isolation / purification Tissue - Human Artery / Aorta

Experiment
RNA isolation / purification Tissue - Human Artery / Aorta
Product
RNeasy Micro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.
Downstream tips
Always blank the spectrophotometer with your elution buffer.
When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

Total RNA extraction from blood/cultured cells and tissue was performed with a spin column-based PAXgene Blood RNA Kit/RNeasy Micro Kit (Qiagen, Hilden, Germany) and phenol-based kit (TRIzol; Invitrogen, Carlsbad, CA, USA). RNA samples were purified by exposure to DNAse I enzyme to digest genomic DNA. High RNA purity was confirmed via microfluidic-based electrophoresis (Agilent 2100 Bioanalyzer platform; Agilent Technologies, Santa Clara, CA, USA).

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Artery / Aorta using RNeasy Micro Kit from Qiagen.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Micro Kit below.

Download PDF Download manufacturer protocol

Videos

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