VWR Life Science RiboZol™ RNA Extraction Reagent

RNA isolation / purification Tissue - Human Gallbladder

Experiment
RNA isolation / purification Tissue - Human Gallbladder
Product
VWR Life Science RiboZol™ RNA Extraction Reagent from VWR
Manufacturer
VWR

Protocol tips

Protocol tips
- If the aqueous layer appears pink, not enough RobiZol was used
Downstream tips
- For RT-PCR applications, DNase treatment might be required.

Publication protocol

Total RNA was extracted with RiboZol™(Amresco, USA) and quantified usingNanoDrop Lite spectrophotometer (ThermoScientific, India). Reverse-Transcriptase PCRwas carried out using Revert Aid First StrandcDNA Synthesis Kit (Thermo Scientific, India)and oligo(dT) primer as per the manufactur-er’s protocol. For every reaction of cDNA syn-thesis 1μg of purified RNA was used followedby PCR amplification using specific primers tocandidate genes listed in Table 1 within thelinear range of amplification (35 cycles). A 10μL aliquot of the amplified product was loadedon 1.6% agarose gel and bands obtained wereanalyzed using ImageJ 1.46r software. The nor-malized gene expression with a >1.5 foldincrease and a P<0.01 was considered to besignificant.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

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Manufacturer protocol

Download the product protocol from VWR for VWR Life Science RiboZol™ RNA Extraction Reagent below.

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