RNeasy Plus Micro Kit

RNA isolation / purification Tissue - Human Gut

Experiment
RNA isolation / purification Tissue - Human Gut
Product
RNeasy Plus Micro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.
Downstream tips
- Always blank the spectrophotometer with your elution buffer.

- When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

Total RNA was isolated using RNeasy® Plus Micro Kit (Qiagen). Maximal amounts of RNA were encoded by reverse transcriptase (Platinum® Taq DNA Polymerase, Invitrogen, Karlsruhe, Germany) and gene expression analysis conducted.

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Gut using RNeasy Plus Micro Kit from Qiagen.

Paper title
Age-related gene expression analysis in enteric ganglia of human colon after laser microdissection
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Micro Kit below.

Download PDF Download manufacturer protocol

Videos

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