MagNA Pure LC RNA Isolation Kit III

RNA isolation / purification Tissue - Human ileum

Experiment
RNA isolation / purification Tissue - Human ileum
Product
MagNA Pure LC RNA Isolation Kit III from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Upstream tips
- The MagNA Pure LC 2.0 Instrument, together with its software is required for analysing the yields of this kit.

- Do not use more sample than this kit is designed to handle.

- Do not allow Wash Buffer I or the Lysis/Binding Buffer to mix with sodium hypochlorite (bleach) solution. This mixture can produce a highly toxic gas.

Publication protocol

The biopsies were homogenized using the MagNA Lyser instrument and MagNa Lyser Green Beads tubes (Roche Applied Science, Mannheim, Germany). Total RNA was extracted from 10 mg of human fresh-frozen intestinal biopsies using the MagNA Pure LC RNA Isolation Kit III (Tissue) and the MagNA Pure LC instrument (Roche), and eluted into RNAse-free water. To generate complementary DNA (cDNA), 2 μg total RNA was subjected to reverse transcriptase-PCR.

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human ileum using MagNA Pure LC RNA Isolation Kit III from Roche Lifesciences.

Paper title
Reduced expression of aquaporins in human intestinal mucosa in early stage inflammatory bowel disease
Full paper
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Manufacturer protocol

Download the product protocol from Roche Lifesciences for MagNA Pure LC RNA Isolation Kit III below.

Download PDF Download manufacturer protocol

Videos

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