Isolate II RNA Mini Kit

RNA isolation / purification Tissue - Human Intestine

Experiment
RNA isolation / purification Tissue - Human Intestine
Product
Isolate II RNA Mini Kit from Bioline
Manufacturer
Bioline

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from up to 1x10^6 cells

Publication protocol

Total cellular RNA was extracted from one centimetre tissue segments using an “Isolate RNA Mini Kit” (Bioline, Luckenwalde, Germany) following the manufacturer’s protocol. The contaminating genomic DNA was digested with DNase I (Invitrogen, Karlsruhe, Germany). RNA concentration was measured spectrophotometrically using an infinite M200 micro plate reader (Tecan, Mainz-Kastel, Germany).



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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

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Manufacturer protocol

Download the product protocol from Bioline for Isolate II RNA Mini Kit below.

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