Maxwell® 16 LEV simplyRNA Purification Kit

RNA isolation / purification Tissue - Human Muscles

Experiment
RNA isolation / purification Tissue - Human Muscles
Product
Maxwell® 16 LEV simplyRNA Purification Kit from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from 5 million cells

Publication protocol

For RNA extraction, the Maxwell 16 LEV simplyRNA Tissue Kit was used (Promega), following the manual's instructions. Briefly, minced and ground human muscle tissue was incubated in 200 µl of chilled 1-Thioglycerol/Homogenization solution and further homogenized using the Precellys 24 system (see DNA). Afterwards, the samples were heated at 70°C for 2 min, then the lysates were allowed to cool down. 200 µl of lysis buffer was added to the cooled-down homogenate, mixed vigorously, followed by transfer of the total 400 µl into well 1 of the Maxwell 16 LEV cartridge. 5 µl of DNAse was added to well 4 of the cartridge and, 50 µl RNAse-free water was added to 0.5 ml Elution Tubes and the RNA extraction program was started at the Maxwell 16 instrument.

Full paper   Login or join for free to view the full paper.

Reviews

Maxwell® 16 LEV simplyRNA Purification Kit from Promega has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Muscles using Maxwell® 16 LEV simplyRNA Purification Kit from Promega.

Paper title
Clinical Classification of Cancer Cachexia: Phenotypic Correlates in Human Skeletal Muscle
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Promega for Maxwell® 16 LEV simplyRNA Purification Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Human Muscles using Maxwell® 16 LEV simplyRNA Purification Kit from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms