TRI Reagent® Sigma

RNA isolation / purification Tissue - Human Muscles

Experiment
RNA isolation / purification Tissue - Human Muscles
Product
TRI Reagent® Sigma from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA

- The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

Skeletal muscle tissue (~30 mg) was immersed in Tri-Reagent (Sigma-Aldrich, MO, United States) in MagNA Lyser 1.4 mm beaded tubes (MagNA Lyser Green Beads, Roche, Germany) and homogenised for 40 secs at 6 m/s in a MagNA Lyser Homogeniser (Roche, Germany), before being stored on ice for 5 mins. This step was repeated three times to ensure complete disruption of muscle tissue sample. RNA was extracted using standard Tri-Reagent procedure via chloroform/isopropanol extractions and 75% ethanol washing as per manufacturer’s instructions.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Muscles using TRI Reagent® Sigma from Sigma-Aldrich.

Paper title
Human Skeletal Muscle Possesses an Epigenetic Memory of Hypertrophy
Full paper
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® Sigma below.

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Videos

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