PicoPure™ RNA Isolation Kit

RNA isolation / purification Tissue - Human Nose

Experiment
RNA isolation / purification Tissue - Human Nose
Product
PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- Can be used when there is a very less amount of starting material

Publication protocol

The tissue was transferred to the lysis buffer from the Arcturus PicoPure RNA extraction kit (ThermoFisher Scientific) for cDNA synthesis, and then to Trizol (Invitrogen) for homogenisation and total RNA extraction according to the manufacturer's instructions. Single‐strand cDNA was generated using Invitrogen's Superscript III First‐Strand Synthesis System kit.

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Nose using PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific.

Paper title
Evidence for a Notch1‐mediated transition during olfactory ensheathing cell development
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PicoPure™ RNA Isolation Kit below.

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Videos

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