PicoPure™ RNA Isolation Kit

RNA isolation / purification Tissue - Human Prostate

Experiment
RNA isolation / purification Tissue - Human Prostate
Product
PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- Can be used when there is a very less amount of starting material

Publication protocol

Total RNA was extracted from LCM captured material using the Arcturus® PicoPure® RNA Isolation Kit (Life Technologies, Grand Island, NY) according to the manufacturer’s protocol. Following tissue dissection, the LCM caps were incubated for 30 min at 42°C in 50 μl of extraction buffer and stored at -20°C until ready for RNA extraction. Cellular lysates from successive slides were pooled into a single tube and mixed with 1 volume of ethanol (70%). The lysate/ethanol solution was loaded onto a high recovery MiraCol™ column and centrifuged at 1000 xg for 2 min followed by 16,000 xg for 30 sec. RNA was recovered in an 11 μl volume. RNA concentration and quality were measured using an Agilent RNA 6000 Pico chip with the Bioanalyzer 2100 (Agilent Technologies) using the manufacturer’s instructions. All samples used for qRT-PCR displayed RNA of good quality as assessed by its RNA Integrity Number (RIN) and of sufficient quantity to not require a pre amplification step for subsequent PCR analysis

Full paper   Login or join for free to view the full paper.

Reviews

PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Prostate using PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific.

Paper title
Dual Action of miR-125b As a Tumor Suppressor and OncomiR-22 Promotes Prostate Cancer Tumorigenesis
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PicoPure™ RNA Isolation Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Human Prostate using PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms