PureLink™ RNA Mini Kit

RNA isolation / purification Tissue - Human Subcutaneous

Experiment
RNA isolation / purification Tissue - Human Subcutaneous
Product
PureLink™ RNA Mini Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- Depending on the material you want to isolate RNA from, please refer to the manual for additional reagents/lab equipment that are required
Downstream tips
- DNase is not supplied with this kit, a DNase kit is available which is compatible with this kit

Publication protocol

Approximately 100 mg of sWAT was used for extraction of RNA using a pure link RNA isolation mini kit (Life Technologies, Carlsbad, CA). cDNA was synthesized and amplified using a high capacity RNA-cDNA kit and TaqManpre Amp master mix kit, respectively (Life Technologies, Carlsbad, CA). Quantitative real-time PCR analyses were performed on an ABI PRISM 7900HT using standard TaqMan master-mix reagents and gene-specific primer assays (Life Technologies, Carlsbad, CA). GAPDH was used as the housekeeping gene to normalize the expression of the target gene. sWAT from healthy children and adults was used as a control to calculate the delta CT values and the fold change.

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Subcutaneous using PureLink™ RNA Mini Kit from Thermo Fisher Scientific.

Paper title
Browning of Subcutaneous White Adipose Tissue in Humans
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PureLink™ RNA Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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