TRIzol Reagent

RNA isolation / purification Tissue - Human Tongue

Experiment
RNA isolation / purification Tissue - Human Tongue
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Tongue and cheek saliva samples were collected using an in-house developed method (unpublished data). Participants were instructed to gently scrape their teeth back and forth across the front of the tongue or gently scrape the teeth over the cheeks and expectorate the saliva into a 15ml centrifuge tube (total 2ml). Centrifuge tubes were kept on ice during the collection procedure (2-10min) and were snap frozen in dry ice and stored at -80°C. To extract the RNA, samples were thawed on ice and 2ml of PBS added and mixed well to dilute the salivary mucins. The samples were then centrifuged (850rcf, 5min), the supernatant discarded and 1ml of TRIzol added to the samples.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Tongue using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue
Full paper
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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Videos

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