RNeasy Lipid Tissue Mini Kit

RNA isolation / purification Tissue - Mouse Hippocampus

Experiment
RNA isolation / purification Tissue - Mouse Hippocampus
Product
RNeasy Lipid Tissue Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- If the RNA yield is low, repeat RNA elution, but incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging.

- All centrifugation steps should be performed at 15–25°C except for phase separation

Publication protocol

RNA preparation was carried out using the Lipid Tissue Mini Kit (Qiagen) following the manufacturer׳s instructions. Hippocampal tissue samples were lysed and homogenized using a handheld rotor-stator homogenizer (Tissue Ruptor, Qiagen) with disposable probes. All centrifugation steps were carried out at 10,000xg and RNA was finally eluted in 50 µl RNase free H2O. After preparation the quantity and quality of the eluted RNA was checked using Nanodrop (Thermo Scientific). Prior to RNA hybridization to the Microarray Chips the RNA quality was tested again using the Bioanaylzer 2100 (Agilent Technology)

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Hippocampus using RNeasy Lipid Tissue Mini Kit from Qiagen.

Paper title
Gender specific hippocampal whole genome transcriptome data from mice lacking the Ca2.3 R-type or Ca3.2 T-type voltage-gated calcium channel
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Lipid Tissue Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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