NucleoSpin® RNA

RNA isolation / purification Tissue - Mouse Prostate

Experiment
RNA isolation / purification Tissue - Mouse Prostate
Product
NucleoSpin® RNA from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Protocol tips
- Aliquot rDNase and store at -20 °C.
Downstream tips
Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

Total RNAs were isolated using NucleoSpin RNA II column kit (Macherey-Nagel, Hoerd, France). cDNAs were synthesized with Moloney Murine Leukemia Virus Reverse Transcriptase (Promega) and random hexamer primers (Promega) according to the manufacturer's instructions. cDNA templates were amplified by MESA GREEN MasterMix Plus for SYBR Assay (Eurogentec, Seraing, Belgium) using an iCycler (Bio-Rad, Marnes-la-Coquette, France). Primer sequences are listed in Text S1. qPCR results were normalized alternatively using 36b4 or 18S as a standard.

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Prostate using NucleoSpin® RNA from Macherey Nagel.

Paper title
Liver X Receptors Protect from Development of Prostatic Intra-Epithelial Neoplasia in Mice
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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® RNA below.

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Videos

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