RNAqueous™ Total RNA Isolation Kit

RNA isolation / purification Tissue - Mouse Uterus

Experiment
RNA isolation / purification Tissue - Mouse Uterus
Product
RNAqueous™ Total RNA Isolation Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from 1 x 10^2–1 x 10^7

Publication protocol

For RNA isolation and gene expression studies, immediately homogenize one of the lanced endometriotic lesions (or ~20 μg uterine tissue) in lysis binding solution and store at -80°C for future RNA isolation with the RNAqueous kit (Ambion) or other method as desired.

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Uterus using RNAqueous™ Total RNA Isolation Kit from Thermo Fisher Scientific.

Paper title
Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for RNAqueous™ Total RNA Isolation Kit below.

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Videos

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