RNeasy Plus Micro Kit

RNA isolation / purification Tissue - Rat Adipose

Experiment
RNA isolation / purification Tissue - Rat Adipose
Product
RNeasy Plus Micro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- When processing <500 cells or <2 µg tissue, carrier RNA may be added to the lysate. Foaming can be reduced by adding Reagent DX at a final concentration of 0.5% (v/v) before disruption and homogenization.

- Add 4 volumes of ethanol (96–100%) to Buffer RPE for a working solution.
before homogenization

Publication protocol

Cells located in the medial and lateral portions of the arcuate nucleus were individually captured into the lids of 0.2 ml PCR tubes containing 25 μl of lysis buffer (RNeasy plus micro kit; Qiagen). The lysis buffer volume was increased by 350 μl and the samples vortexed for 30 s and frozen at −80°C until RNA extraction (RNeasy plus micro kit; Qiagen). The RNA content of each sample was determined using a Nanodrop ND-1000 spectrophotometer and standardized; only samples with 260/280 readings between 1.60 and 2.00 were used for analysis. The RNA from each sample was then reverse transcribed using a Superscript kit (Superscript VILO; Invitrogen).

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Adipose using RNeasy Plus Micro Kit from Qiagen.

Paper title
Neurochemical characterization and sexual dimorphism of projections from the brain to abdominal and subcutaneous white adipose tissue in the rat.
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Micro Kit below.

Download PDF Download manufacturer protocol

Videos

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