TRIzol Reagent

RNA isolation / purification Tissue - Rat Bone marrow

Experiment
RNA isolation / purification Tissue - Rat Bone marrow
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Nasal epithelial tissue samples were disrupted and homogenized using a TissueRuptor (Qiagen) and RNA isolated using the miRNeasy kit (Qiagen). For the WBC, samples were homogenized in TRIzol (Life Technologies) and RNA isolated according to the standard TRIzol protocol. The BM samples were filtered through 70-μm nylon mesh filters (Fischer Scientific, Waltham, Massachusetts) to remove bone fragments. Filtered BM samples were then homogenized in TRIzol (Life Technologies) and RNA isolated according to the standard TRIzol protocol. Extracted RNA was quantified with a Nanodrop 1000 spectrophotometer (Thermo Scientific, Waltham, Massachusetts) and its integrity verified with a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, California) using both the Nano and Small RNA kits. The quality control incorporated RNA integrity number assessment and electrophoretogram peak assessment of small RNA molecules.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Bone marrow using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Formaldehyde-Associated Changes in microRNAs: Tissue and Temporal Specificity in the Rat Nose, White Blood Cells, and Bone Marrow
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download PDF Download manufacturer protocol

Videos

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