High Pure RNA Isolation Kit

RNA isolation / purification Tissue - Rat Liver

Experiment
RNA isolation / purification Tissue - Rat Liver
Product
High Pure RNA Isolation Kit from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Upstream tips
- Be careful to create an RNase-free working environment
Protocol tips
- Always mix the sample tube well after addition of each reagent.

Publication protocol

Total RNA was isolated from 5 mg liver tissue using High Pure RNA isolation kit (Roche, Germany). According to the handbook kit, the samples were treated with DNase I enzyme supported in the kit to avoid DNA contamination. Finally, Optical Density (A260/A280 and A260/A230) and concentration were measured using Nanophotometer 2000c (Thermo Science, USA).

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Liver using High Pure RNA Isolation Kit from Roche Lifesciences.

Paper title
Expression of and Genes in MDMA-induced Hepatotoxicity on Rat Liver Using Quantitative Real-Time PCR Method through Triggering Programmed Cell Death
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Manufacturer protocol

Download the product protocol from Roche Lifesciences for High Pure RNA Isolation Kit below.

Download PDF Download manufacturer protocol

Videos

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