GenElute™ Mammalian Total RNA Miniprep Kit

RNA isolation / purification Tissue - Rat Ovaries

Experiment
RNA isolation / purification Tissue - Rat Ovaries
Product
GenElute™ Mammalian Total RNA Miniprep Kit from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
- Samples require immediate and thorough disruption in the Lysis Solution/2-ME Mixture to prevent degradation of RNA.

Publication protocol

total RNA was isolated according to the manufacturer's instructions using the RNeasy Mini Kit (Qiagen), AllPrep RNA/Protein kit, GenElute Mammalian Total RNA Miniprep Kit (Sigma- Aldrich), or TRIzol Reagent (Life Technologies), followed by further RNA purification with the RNeasy kit.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Ovaries using GenElute™ Mammalian Total RNA Miniprep Kit from Sigma-Aldrich.

Paper title
Simultaneous isolation of mRNA and native protein from minute samples of cells.
Full paper
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for GenElute™ Mammalian Total RNA Miniprep Kit below.

Download PDF Download manufacturer protocol

Videos

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