RNAGEM™ Tissue PLUS

RNA isolation / purification Tissue - Rat Pineal gland

Experiment
RNA isolation / purification Tissue - Rat Pineal gland
Product
RNAGEM™ Tissue PLUS from ZyGEM
Manufacturer
ZyGEM

Protocol tips

Downstream tips
- Absorbance 260/280 nm is an ineffective quantitation method with RNAGEM-prepared nucleic acids

Publication protocol

For qRT-PCR analysis of the effect of Crx-sh on Aanat mRNA levels in cultured rat pinealocytes, pinealocyte samples were centrifuged and total RNA was extracted using RNAGEMM Tissue (ZyGEM) according to the instruction of the manufacturer.

Full paper   Login or join for free to view the full paper.

Reviews

RNAGEM™ Tissue PLUS from ZyGEM has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Pineal gland using RNAGEM™ Tissue PLUS from ZyGEM.

Paper title
Circadian dynamics of the cone-rod homeobox (CRX) transcription factor in the rat pineal gland and its role in regulation of arylalkylamine N-acetyltransferase (AANAT).
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from ZyGEM for RNAGEM™ Tissue PLUS below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Rat Pineal gland using RNAGEM™ Tissue PLUS from ZyGEM. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms