QIAamp DNA Blood Mini Kit

DNA isolation / purification Tissue - eye

Experiment
DNA isolation / purification Tissue - eye
Product
QIAamp DNA Blood Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA extraction.
For ocular samples, we extracted toxoplasmic DNA from up to 10 μl of the ocular sample using the QIAmp DNA Blood Minikit (Qiagen). For blood, we used two different extraction protocols. The initial protocol was performed using 200 μl of whole blood and the QIAmp DNA Blood Minikit. We followed this up with a second protocol using 2 ml of whole blood and the QIAmp DNA Blood Midi kit (Qiagen). PCR performed with extract retrieved from 200 μl of whole blood was called SV-PCR (small volume), and that performed with extract retrieved from 2 ml of blood was named LV-PCR (large volume). DNA was eluted with 100 μl of elution solution for SV-PCR and 300 μl for LV-PCR. The albumin gene was amplified as a control for extraction. After DNA extraction, samples were kept at +4°C for up to 48 h or frozen at −35°C for use within the week.

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Reviews

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Discussion

Discussion

5 years ago

Author: Denmark

What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Tissue - eye using QIAamp DNA Blood Mini Kit from Qiagen.

Paper title
PCR-Based Detection of DNA in Blood and Ocular Samples for Diagnosis of Ocular Toxoplasmosis
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Blood Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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