Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
"The MagNA Pure LC 2.0 Instrument, together with its software is required for analysing the yields of this kit.
Do not use more sample than this kit is designed to handle.
Do not allow Wash Buffer I or the Lysis/Binding Buffer to mix with sodium hypochlorite (bleach) solution. This mixture can produce a highly toxic gas" |
|
|
Upstream tips |
"The MagNA Pure LC 2.0 Instrument, together with its software is required for analysing the yields of this kit.
Do not use more sample than this kit is designed to handle.
Do not allow Wash Buffer I or the Lysis/Binding Buffer to mix with sodium hypochlorite (bleach) solution. This mixture can produce a highly toxic gas" |
Publication protocol
RNA isolation from lung tissue with additional determination of RNA concentrations and integrity was performed as described previously.12 RNA from cells in the dLNs was isolated using a MagNA Pure LC RNA Isolation High Performance kit and MagNA Pure System (Roche, Basel, Switzerland). For lung tissue, RNA concentrates from naive and PM mice were analyzed as individual samples, whereas those of SC mice were pooled
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Papers
Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Bordetella pertussis using MagNA Pure LC RNA Isolation Kit from Roche Lifesciences.
Paper title
Molecular and cellular signatures underlying superior immunity against Bordetella pertussis upon pulmonary vaccination.
Videos
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