Direct-zol™ RNA MiniPrep Plus

RNA isolation / purification Bacteria - Gram negative Borrelia burgdorferi

Experiment
RNA isolation / purification Bacteria - Gram negative Borrelia burgdorferi
Product
Direct-zol™ RNA MiniPrep Plus from Zymo Research
Manufacturer
Zymo Research

Protocol tips

Upstream tips
Tough-to-lyse samples (i.e.,gram-positive bacteria, yeast, fungi, animal tissue, plant, etc.) can be homogenized in TRI Reagent® (supplied with kit) with a mortar/pestle, dounce, tissue grinder, or bead beating with a high-speed homogenizer prior to RNA Purification.

Publication protocol

RNA was isolated from 500 μl of cell suspension using the Zymo RNA Direct-Zol miniprep kit (Zymo). Isolated RNA was eluted in 35 μl RNase-free water and stored at -80C. RNA quantity and integrity was assayed by microfluidic analysis using an Agilent 2100 Bioanalyzer and with the Agilent RNA 6000 Nano chip kit (Agilent) and only samples with a RIN score > 9 were used for library construction.

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Discussion

Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Borrelia burgdorferi using Direct-zol™ RNA MiniPrep Plus from Zymo Research.

Paper title
RNA-Seq of in Multiple Phases of Growth Reveals Insights into the Dynamics of Gene Expression, Transcriptome Architecture, and Noncoding RNAs
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Manufacturer protocol

Download the product protocol from Zymo Research for Direct-zol™ RNA MiniPrep Plus below.

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