VWR Life Science RiboZol™ RNA Extraction Reagent

RNA isolation / purification Bacteria - Gram negative Legionella pneumophilia

Experiment
RNA isolation / purification Bacteria - Gram negative Legionella pneumophilia
Product
VWR Life Science RiboZol™ RNA Extraction Reagent from VWR
Manufacturer
VWR

Protocol tips

Protocol tips
If the aqueous layer appears pink, not enough RobiZol was used
Downstream tips
For RT-PCR applications, DNase treatment might be required

Publication protocol

Total L. pneumophila RNA was extracted from cells at an OD620 of 0.9 by the RiboZol RNA extraction reagent (Amresco, OH). Total RNA was treated with DNase I, and RNA concentrations were determined using a NanoDrop ND-1000 spectrophotometer (Nanodrop Technologies). First-strand cDNA was generated using the SuperScript first-strand synthesis system for reverse transcriptase PCR (RT-PCR) (Invitrogen, CA). When the RT reaction was completed, samples were adjusted to 200 μl with molecular biology water.

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Discussion

Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Legionella pneumophilia using VWR Life Science RiboZol™ RNA Extraction Reagent from VWR.

Paper title
Disulfide Bond Oxidoreductase DsbA2 of Exhibits Protein Disulfide Isomerase Activity
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Manufacturer protocol

Download the product protocol from VWR for VWR Life Science RiboZol™ RNA Extraction Reagent below.

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