TRI Reagent™ Solution

RNA isolation / purification Bacteria - Gram negative Salmonella typhi

Experiment
RNA isolation / purification Bacteria - Gram negative Salmonella typhi
Product
TRI Reagent™ Solution from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Carefully watch how much TRI reagent should be added to your samples

Publication protocol

Inner and outer foreskin pieces and colon biopsies were preserved in RNAlater (Life Technologies) and stored at −80°C. RNA isolations were carried out by placing the thawed tissue in 1ml of TRI Reagent (Life Technologies) and homogenizing it with a homogenizer with wide probe (Omni). Nucleic acid separation used 1-bromo-3-chloropropane (Sigma-Aldrich) and Ribopure Kit (Life Technologies) columns. Blood was collected in Tempus tubes, with RNA isolated according to the Tempus Spin RNA Isolation Kit (Life Technologies).



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Discussion

Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Salmonella typhi using TRI Reagent™ Solution from Thermo Fisher Scientific.

Paper title
In Men at Risk of HIV Infection, IgM, IgG1, IgG3 and IgA Reach the Human Foreskin Epidermis
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRI Reagent™ Solution below.

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Videos

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