REzol C&T

RNA isolation / purification Bacteria - Gram positive Clostridium difficile

Experiment
RNA isolation / purification Bacteria - Gram positive Clostridium difficile
Product
REzol C&T from PROtech
Manufacturer
PROtech

Protocol tips

Protocol tips
Total RNA was extracted using REzol (PROtech, Taipei, Taiwan). mRNA levels were analyzed with real-time quantitative RT-PCR (Applied Biosystems, Foster City, CA) with Actb as the reference gene in each reaction

Publication protocol

Total RNA was extracted using REzol (PROtech, Taipei, Taiwan). mRNA levels were analyzed with real-time quantitative RT-PCR (Applied Biosystems, Foster City, CA) with Actb as the reference gene in each reaction

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Discussion

Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram positive Clostridium difficile using REzol C&T from PROtech.

Paper title
The ATP-P2X Signaling Axis Is an Essential Sentinel for Intracellular Pathogen-Induced Inflammasome Activation
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Manufacturer protocol

Download the product protocol from PROtech for REzol C&T below.

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Videos

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