RNeasy Plus Micro Kit

RNA isolation / purification Tissue - Human Liver

Experiment
RNA isolation / purification Tissue - Human Liver
Product
RNeasy Plus Micro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- When processing <2 µg tissue, carrier RNA may be added to the lysate. Foaming can be reduced by adding Reagent DX at a final concentration of 0.5% (v/v) before disruption and homogenization.

- Add 4 volumes of ethanol (96–100%) to Buffer RPE for a working solution.
before homogenization

Publication protocol

Besides mRNA extraction from the FACS and LMD groups, 10-μm-thick whole liver tissue slides, sectioned from frozen tissue, were homogenised and lysed in RTL plus buffer with 1% 2-mercaptoethanol. Total mRNA from whole liver, FACS and microdissection samples was extracted using the RNeasy Plus Micro Kit (Qiagen) according to the manufacturer’s instructions.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Micro Kit below.

Download PDF Download manufacturer protocol

Videos

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