SV Total RNA Isolation System

RNA isolation / purification Tissue - Human Liver

Experiment
RNA isolation / purification Tissue - Human Liver
Product
SV Total RNA Isolation System from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
- Do not freeze-thaw the DNase more than three times after rehydration

Publication protocol

Hepatic tissue samples were collected using RNAlater ® (Thermo Fisher Scientific, Inc., CA, USA) and stored at -70oC until ribonucleic acid (RNA) extraction. RNA from hepatic tissue was isolated using the spin (SV) Total RNA Isolation System kit (Promega Corporation, Madison, WI, USA), according to the manufacturer’s specifications.



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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Liver using SV Total RNA Isolation System from Promega.

Paper title
Platelet-derived growth factor A mRNA in platelets is associated with the degree of hepatic fibrosis in chronic hepatitis C.
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Manufacturer protocol

Download the product protocol from Promega for SV Total RNA Isolation System below.

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Videos

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