TRI Reagent® MRC

RNA isolation / purification Tissue - Rat Heart

Experiment
RNA isolation / purification Tissue - Rat Heart
Product
TRI Reagent® MRC from Molecular Research Center, Inc.
Manufacturer
Molecular Research Center, Inc.

Protocol tips

Upstream tips
-1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA

- The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

Total RNA was extracted from rat neonatal CMs, cardiac fibroblasts and hearts using TRI Reagent (Sigma-Aldrich) and was resolved in sterilized RNase-free water. The RNA concentration was determined by NanoDrop spectrophotometer (Thermo Scientific Inc., Waltham, MA) and the purified RNA was aliquot and stored at −80 °C. exRNA in culture media or mouse sera was extracted using Trizol LS and quantified using Quant-iTTM RNA assay kit (Life Technologies, Grand Island, NY).

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

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Manufacturer protocol

Download the product protocol from Molecular Research Center, Inc. for TRI Reagent® MRC below.

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