LC3B Antibody

Protocol tips

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	Dilute primary Ab at 1:400 and incubate overnight

Protocol tips
Dilute primary Ab at 1:400 and incubate overnight

Publication protocol

Immunofluorescence analysis for LC3 was performed, as described previously (18). Briefly, cells were fixed in 4% paraformaldehyde at 4°C for 10 min. They were then permeabilized with 0.01% Triton X-100, before being blocked with 10% BSA for 1 h. Cells were stained with primary Ab (rabbit anti-human LC3, diluted 1:400) overnight, and then incubated with secondary Ab (anti-rabbit IgG-tetramethylrhodamine isothiocyanate or anti-rabbit IgG-Alexa488) for 1 h. Unbound Ab was removed with PBS, and cells were imaged using a Zeiss LSM510 META confocal microscope (Zeiss).

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Manufacturer protocol

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