FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme

TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells

Experiment
TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells
Product
FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme from Millipore
Manufacturer
Millipore

Protocol tips

Upstream tips
Precoating slides with poly-L-lysine may enhance cell adherence.

Do not allow the samples to dry out.

All other FragELTM kit components, with the exception of the Mounting Media, should be kept on ice during usage then promptly returned to -20C
Protocol tips
Dilute 5X TdT Equilibration Buffer 1:5 with water.

Place slides in a humidified chamber and incubate at 37C for 1 - 1.5 hours after ading TdT Labeling Reaction Mixture

Publication protocol

The MTT assays were performed after transfection for 0, 24, 48, and 96 hours as described previously (45).

Flow cytometric analysis was conducted at the Flow Cytometry facility of Peking Union Medical College. Briefly, after 48 to 72 hours of transfection, the cells were fixed and then incubated in PBS containing Rnase A (100 μg/ml; Sigma-Aldrich) and propidium iodide (50 μg/ml; Sigma-Aldrich) at room temperature for 30 minutes prior to analysis.

Glioma cells were infected with siRNAs or plasmids in 24-well plates for 48 to 72 hours. Then, the TUNEL assay was performed using a FragEL DNA fragmentation detection kit (Merck) according to the manufacturer’s instructions.

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells using FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme from Millipore.

Paper title
RNA-binding protein PCBP2 modulates glioma growth by regulating
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Manufacturer protocol

Download the product protocol from Millipore for FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme below.

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