ApoAlert™ DNA Fragmentation Assay Kit

TUNEL assay cell type - Islets of langerhans (Beta cells)

Experiment
TUNEL assay cell type - Islets of langerhans (Beta cells)
Product
ApoAlert™ DNA Fragmentation Assay Kit from Takara Bio Inc
Manufacturer
Takara Bio Inc

Protocol tips

Protocol tips
Fix cells in 4% paraformaldehyde and incubated in 0.3% H2O2 in methanol for five minutes to block endogenous peroxidase.

After TdT treatment incubate cells at 37°C incubator for 60 min.

Publication protocol

The detection of apoptotic nuclei using the TUNEL staining was performed with ApoAlert™ DNA Fragmentation Assay Kit (Clonetech, Palo Alto, CA). Briefly, cells were fixed in 4% paraformaldehyde and incubated in 0.3% H2O2 in methanol for five minutes to block endogenous peroxidase. The slides were then incubated with fluorescent-conjugated avidin in PBS for two hours at room temperature. After three washes, slides were cover slipped with glycerol phosphate buffer. Apoptotic cells were counted in at least 10 random fields and expressed as a percentage of the total cell number (apoptotic index).

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

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Manufacturer protocol

Download the product protocol from Takara Bio Inc for ApoAlert™ DNA Fragmentation Assay Kit below.

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