ApopTag® Fluorescein In Situ Apoptosis Detection Kit

TUNEL assay cell type - Islets of langerhans (Beta cells)

Experiment
TUNEL assay cell type - Islets of langerhans (Beta cells)
Product
ApopTag® Fluorescein In Situ Apoptosis Detection Kit from Millipore
Manufacturer
Millipore

Protocol tips

Protocol tips
Apply protinease K for 15 min

Apply TdT enzyme and incubate in a humidified chamber at 37°C for 1 hour.

Publication protocol

The TUNEL assay was performed to detect apoptosis in the islets. The ApopTag Fluorescein in Situ Apoptosis Detection Kit (Chemicon International, Temecula, CA) was used according to the manufacturer's instructions. Fifteen samples were obtained by three independent experiments in each group. TUNEL+ cells and all cells were counted to calculate the ratio of DAPI+ cells

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Reviews

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - Islets of langerhans (Beta cells) using ApopTag® Fluorescein In Situ Apoptosis Detection Kit from Millipore.

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Manufacturer protocol

Download the product protocol from Millipore for ApopTag® Fluorescein In Situ Apoptosis Detection Kit below.

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